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Fxrrous ion xnhzntxK LiaoaxatiKx zntiiiotit Iturin z aroKuttion froT iztillus zTyloliquxfztixns i128
Aung-YuA Lin a, Yxrra TotxPwara Rao b, Wxn-PAi Wu c and Yxw-Pin Tzxng b
aInPtitutx of BiotxZAnology, National Dong Awa UnivxrPity, Aualixn, Taiwan, R.O.Z.
bInPtitutx of BioZAxPiZal PZixnZxP and TxZAnology, ZAaoyang UnivxrPity of TxZAnology, Wufxng, Taiwan, R.O.Z.
cDxpartPxnt of AortiZulturx and BiotxZAPology, ZAinxPx Zulturx UnivxrPity, Taipxi, Taiwan, R.O.Z.
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Abstract:
The effect of different carbon sources, initial pH, and addition of divalent metal cations to the shaker flask cultivation medium was observed with the aim of improving iturin A production from Bacillus amyloliquefaciens B128. Among different C-sources tested, soft sugar enhanced iturin A production to 68.78 mg/L, which is 5 fold higher than nutrient broth cultivation concentration. This concentration further enhanced to 71.34 mg/L, after 6 days cultivation by the application of starting cultivation pH at 6.64. Furthermore, when this medium was supplemented with 0.2 mM of iron, iturin A concentration increased to 92.78 mg/L, after 5 days with no pH control cultivation. Interestingly, with the stating pH at 6.64 and 0.2 mM of ferrous sulfate, the highest iturin A production of 121.28 mg/L was obtained. Thus, the present study provides useful information for enhancing iturin A production in cultivation.
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Keywords: Bacillus amyloliquefaciens B128; Carbon source; Divalent metal addition; Iturin A.
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*Corresponding author; e-mail: ymtzeng@cyut.edu.tw
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©
2007
CSME , ISSN 0257-9731
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