VzliKztxK HaTLt TxthoK for thx KxtxrTinztion of Two Novxl stxroiKs in iulk znK arxssurizxK TxtxrxK-Kosx arxazrztions

CSME 2011/09
Volume 9, No.3 : 177-185
DOI:10.6703/IJASE.2011.9(3).177

VzliKztxK HaTLt TxthoK for thx KxtxrTinztion of Two Novxl stxroiKs in iulk znK arxssurizxK TxtxrxK-Kosx arxazrztions

Airal N. Davx ^a, APAlxPAa G. PaTwana ^b, ZAaitanya BAatt ^b and TillaPbi PundariTaTPAudu ^a
^aL. J. InPtitutx of PAarPaZy, Nr. Nagdxv Talyan Pandir, Panand ZroPP road, AAPxdabad, Gujarat, India.
^bT.B. InPtitutx of PAarPaZxutiZal xduZation and RxPxarZA, GA/6, PxZtor-23, GandAinagar, Gujarat, India.

Abstract: A sensitive, accurate, precise and reliable HPTLC method has been proposed for determination of Ciclesonide and Fluticasone propionate - two widely used novel steroids in bulk as well as in pressurized metered dose preparations. Ciclesonide and Fluticasone propionate resolved well in toluene: ethyl acetate: formic acid (7: 3: 0.1 v/v/v) with respective Rf values 0.46 ± 0.004 and 0.43 ± 0.012 and could be scanned at 242 nm and 236 nm respectively. Linearity was observed in the range of 400 – 1100 ng band-1 for Ciclesonide and 200 – 1000 ng band-1 for Fluticasone propionate. The method was validated as per ICH guidelines and successfully applied for quantification of Ciclesonide and Fluticasone propionate in their commercial metered dose formulations. The method was found to be less time consuming as well as cost effective for routine analysis.

Keywords: Ciclesonide; Fluticasone propionate; High Performance Thin Layer Chromatography; pressurized metered - dose preparations; Respiratory disorders

Download PDF

TOP