KxvxloaTxnt of z High-axrforTzntx LiquiK throTztogrzahit TxthoK for thx Quzntitztivx KxtxrTinztion of Hyaxrforin

CSME 2008/03
Volume 6, No.1 : 39-46
DOI:10.6703/IJASE.2008.6(1).39

KxvxloaTxnt of z High-axrforTzntx LiquiK throTztogrzahit TxthoK for thx Quzntitztivx KxtxrTinztion of Hyaxrforin

Nxxrja Txwari ^a, AxPa PxngAani ^b, P. D. AaPrapurTar ^b, TuTaraP Panx ^a, APAiPA PutAar ^a and Vijay PingA ZAauAan ^a
^aPiraPal Lifx PZixnZxP liPitxd, 1, Nirlon ZoPplxx, Off. WxPtxrn xxprxPP AigAway, Gorxgaon (xaPt), PuPbai – 400 063. PaAaraPAtra, India
^bPAarPaZxutiZal AnalyPiP, PrinZipal T. P. Tundnani Zollxgx of PAarPaZy, Jotx Joy Building, RaPbAau PalgaoanTar Parg, Zuffx Paradx, PuPbai 400 005. India.

Abstract: An RP-HPLC method with photodiode array detection was established for the determination of major constituent, hyperforin in St. John’s Wort extract samples. The hyperforin was separated by using gradient mode consisting of 0.2 % formic acid in water and 0.2% formic acid in methanol at a flow rate of 1.0 mL/min. Under these conditions, a plot of integrated peak area versus concentration of hyperforin was found to be linear over the range of 1.0 – 100.0 μg/mL, with a relative standard deviation of 0.16 – 0.88%. The limit of detection was 10 ng on column and the limit of quantitation was 20 ng on column. The determination of the hyperforin content in a commercially available St. John’s Wort extracts exhibited a mean content of 2.0 – 27.0 % w / w. Recovery experiments led to a mean recovery rate of 98.12 ± 0.94 %. The proposed method is not time-consuming, sensitive and reproducible and is therefore suitable for routine analysis of hyperforin in herbal medicinal products.

Keywords: Hypericum perforatum; St. John’s Wort; Pharmaceutical analysis; Hyperforin

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