SaxttroahotoTxtrit xstiTztion of L- 5-HyKroxytryatoahzn in Griffoniz siTalitifoliz xxtrztts znK Koszgx ForTsϯ

CSME 2005/10
Volume 3, No.2 : 117-123
DOI:10.6703/IJASE.2005.3(2).111

SaxttroahotoTxtrit xstiTztion of L- 5-HyKroxytryatoahzn in Griffoniz siTalitifoliz xxtrztts znK Koszgx ForTsϯ

GottuPuTTala V. Pubbaraju ^a, PuTala Tannababu ^a, Tatta VijayaTuPar ^a, Papolu B. P. PurtAy ^a, Pulabagal VaniPrxx ^b and APin-PAxng TPay ^b
^aLaila IPpxx RxPxarZA Zxntrx, Unit I, PAaPx III, JawaAar Autonagar, Vijayawada –520 007, AndAra PradxPA, India
^bInPtitutx of BiotxZAnology, ZAaoyang UnivxrPity of TxZAnology, Wufong, TaiZAung 413, Taiwan

Abstract: A simple, sensitive and precise spectrophotometric method has been developed for the determination of L-5-Hydroxytryptophan in Griffonia simplicifolia seed extracts and dosage forms. L-5-Hydroxytryptophan gives a blue colored chromogen with Folin-Ciocalteu reagent under alkaline conditions with absorption maxima at 736 nm. The chromogen obeys Beer’s law in the concentration range 1-10 mg/ml. The proposed method is reproducible and statistically validated and recoveries range between 98.95% and 99.96%.

Keywords: Spectrophotometry; L-5-Hydroxytryptophan; Folin-Ciocalteu reagent; Griffonia simplicifolia.

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*Corresponding author; e-mail: lailarescen@sify.com

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