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CSME 2019/06
Volume 40 No.3
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257-262
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Effects on Protein Preconcentration Using Nano-interstices via Self-assembled Monolayers with Different Concentrations of Au Nanoparticles
Ngoc-Viet Nguyena, Meng-Syuan Wua, Neng-Chuan Tienb and Chun-Ping Jena
aDepartment of Mechanical Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan
bNuclear Science and Technology Development Center, National Tsing Hua University, Hsinchu 30013, Taiwan
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Abstract:
This study aimed to enhance the performance of a self-assembled monolayer of gold nanoparticles for a protein-preconcentration microchip that uses nanofractures generated by junction gap breakdown. Simple and low-cost techniques are applied to fabricate the chip. A mold master for the cross-shaped microchannels is created by the basic photolithography process on a silicon wafer. A suitable biocompatibility polydimethylsiloxane (PDMS) material is prepared and poured onto the mold master to replicate the channels. Subsequently, the glass substrate is silanized prior to functionalizing the gold nanoparticles at the designed region for self-assembly. The electrical breakdown voltage required for nanofracture generation is greatly reduced. The PDMS channel replica is bonded to the glass substrate using oxygen–plasma treatment. In the experiment, the chip is used to collect 10 μM Bovine serum albumin (BSA) labeled with fluorescein isothiocyanate (FITCBSA). Different concentrations of the gold-nanoparticle solution ranging from 1.325 nM to 26.5 nM are investigated. The optimal concentration of gold-nanoparticle solution is determined to be 1.325 nM. Furthermore, the concentration of FITC-labeled BSA at the desired region increases approximately 50 times from 10 μM to 0.5 mM in 15 min. These results demonstrate that a reliable method may be used to increase the concentration efficiency of the proposed chip.
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Keywords: protein preconcentration, nanofracture, self-assembled monolayer, gold nanoparticles.
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©
2019
CSME , ISSN 0257-9731
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